Expression and Secretion of RANTES (CCL5) in Granulomatous Calcified Tissue before and after Lipopolysaccharide Treatment In Vivo
Identifieur interne : 002351 ( Main/Exploration ); précédent : 002350; suivant : 002352Expression and Secretion of RANTES (CCL5) in Granulomatous Calcified Tissue before and after Lipopolysaccharide Treatment In Vivo
Auteurs : M. L. Castellani [Italie] ; L. N. Shanmugham [États-Unis] ; C. Petrarca [Italie] ; I. Simeonidou [Grèce] ; S. Frydas [Grèce] ; M. De Colli [Italie] ; J. Vecchiet [Italie] ; K. Falasca [Italie] ; S. Tetè [Italie] ; V. Salini [Italie] ; P. Conti [Italie]Source :
- Calcified Tissue International [ 0171-967X ] ; 2007-01-01.
English descriptors
- KwdEn :
Abstract
Abstract: RANTES (regulated on activation, normal T cell-expressed and secreted) is a CC chemokine appearing to be involved in the recruitment of leukocytes at inflammation sites. RANTES is produced by CD8+ T cells, epithelial cells, fibroblasts, and platelets. It acts in vitro in leukocyte activation and human immunodeficiency virus suppression, but its role in vivo is still uncertain. In our study, we established the involvement of RANTES in an in vivo model of chronic inflammation induced by potassium permanganate, leading to calcified granulomas. In our rat model, RANTES expression (mRNA and protein) was significantly upregulated in granulomatous tissue; RANTES expression was further increased upon i.p. injection of lipopolysaccharide (LPS), while it was kept at basal levels by dexamethasone (Dex) given 18 hours before sacrifice. LPS and Dex increased and decreased, respectively, the recruitment of mononuclear cells in granulomatous tissue compared with control granulomas from phosphate-buffered saline (PBS)-treated animals. In granuloma tissue, levels of RANTES were higher in LPS-treated rats and lower in the Dex group compared to controls. RANTES was also found in the conditioned medium of granuloma tissue from treated (LPS or Dex) and untreated (PBS) rats. When LPS was added in vitro for 18 hours, RANTES was further increased, except in the Dex group (P > 0.05). On serum analysis, RANTES levels were higher in the LPS group and lower in the Dex group compared to controls. This study shows for the first time that RANTES is produced in vivo in chronic, experimental inflammatory states, an effect increased by LPS and inhibited by Dex.
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DOI: 10.1007/s00223-006-0115-2
Affiliations:
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Conti</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Immunology Division, Department of Oncology and Neuroscience, University of Chieti-Pescara, 66100, Chieti</wicri:regionArea><wicri:noRegion>Chieti</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Calcified Tissue International</title><title level="j" type="abbrev">Calcif Tissue Int</title><idno type="ISSN">0171-967X</idno><idno type="eISSN">1432-0827</idno><imprint><publisher>Springer-Verlag; www.springer-ny.com</publisher><pubPlace>New York</pubPlace><date type="published" when="2007-01-01">2007-01-01</date><biblScope unit="volume">80</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="60">60</biblScope><biblScope unit="page" to="67">67</biblScope></imprint><idno type="ISSN">0171-967X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0171-967X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CCL5</term><term>Calcified granuloma</term><term>In vivo</term><term>Inflammation</term><term>Lipopolysaccharide</term><term>RANTES</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: RANTES (regulated on activation, normal T cell-expressed and secreted) is a CC chemokine appearing to be involved in the recruitment of leukocytes at inflammation sites. RANTES is produced by CD8+ T cells, epithelial cells, fibroblasts, and platelets. It acts in vitro in leukocyte activation and human immunodeficiency virus suppression, but its role in vivo is still uncertain. In our study, we established the involvement of RANTES in an in vivo model of chronic inflammation induced by potassium permanganate, leading to calcified granulomas. In our rat model, RANTES expression (mRNA and protein) was significantly upregulated in granulomatous tissue; RANTES expression was further increased upon i.p. injection of lipopolysaccharide (LPS), while it was kept at basal levels by dexamethasone (Dex) given 18 hours before sacrifice. LPS and Dex increased and decreased, respectively, the recruitment of mononuclear cells in granulomatous tissue compared with control granulomas from phosphate-buffered saline (PBS)-treated animals. In granuloma tissue, levels of RANTES were higher in LPS-treated rats and lower in the Dex group compared to controls. RANTES was also found in the conditioned medium of granuloma tissue from treated (LPS or Dex) and untreated (PBS) rats. When LPS was added in vitro for 18 hours, RANTES was further increased, except in the Dex group (P > 0.05). On serum analysis, RANTES levels were higher in the LPS group and lower in the Dex group compared to controls. This study shows for the first time that RANTES is produced in vivo in chronic, experimental inflammatory states, an effect increased by LPS and inhibited by Dex.</div></front></TEI><affiliations><list><country><li>Grèce</li><li>Italie</li><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="Italie"><noRegion><name sortKey="Castellani, M L" sort="Castellani, M L" uniqKey="Castellani M" first="M. L." last="Castellani">M. L. Castellani</name></noRegion><name sortKey="Conti, P" sort="Conti, P" uniqKey="Conti P" first="P." last="Conti">P. Conti</name><name sortKey="Conti, P" sort="Conti, P" uniqKey="Conti P" first="P." last="Conti">P. Conti</name><name sortKey="De Colli, M" sort="De Colli, M" uniqKey="De Colli M" first="M." last="De Colli">M. De Colli</name><name sortKey="Falasca, K" sort="Falasca, K" uniqKey="Falasca K" first="K." last="Falasca">K. Falasca</name><name sortKey="Petrarca, C" sort="Petrarca, C" uniqKey="Petrarca C" first="C." last="Petrarca">C. Petrarca</name><name sortKey="Salini, V" sort="Salini, V" uniqKey="Salini V" first="V." last="Salini">V. Salini</name><name sortKey="Tete, S" sort="Tete, S" uniqKey="Tete S" first="S." last="Tetè">S. Tetè</name><name sortKey="Vecchiet, J" sort="Vecchiet, J" uniqKey="Vecchiet J" first="J." last="Vecchiet">J. Vecchiet</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Shanmugham, L N" sort="Shanmugham, L N" uniqKey="Shanmugham L" first="L. N." last="Shanmugham">L. N. Shanmugham</name></region></country><country name="Grèce"><noRegion><name sortKey="Simeonidou, I" sort="Simeonidou, I" uniqKey="Simeonidou I" first="I." last="Simeonidou">I. Simeonidou</name></noRegion><name sortKey="Frydas, S" sort="Frydas, S" uniqKey="Frydas S" first="S." last="Frydas">S. Frydas</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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